Purified Anti-Whitlow 218 Linker Antibody[DA011] (AN009990P)

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All	Size	Price	Qty
	100μg	$ 88.00	- +
	25μg	$ 38.00	- +

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Conjugation: Unconjugated

Clone	DA011
Verified Samples	Verified Samples in FCM: HEK293T cells transfected with anti-CD19(FMC63 scFv with Whitlow/218 Linker) CAR
Dilution	FCM ≤ 0.2 µg per million cells in 100 µL volume
Background	The whitlow/218 linker, with the sequence GSTSGSGKPGSGEGSTKG, is a commonly used synthetic peptide linker. Whitlow/218 linker often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The scFv containing the 218 linker showed reduced aggregation and was found to be more stable to proteolysis in vitro.
Immunogen	Synthetic peptide
Abbre	Whitlow 218 Linker
Host	Rabbit
Clonality	Monoclonal
Purification Method	>98%, Protein A/G purified
Isotype	Rabbit IgG
Applications	FCM
Concentration	≥ 1 mg/mL
Conjugation	Unconjugated
Storage Buffer	Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.
Storage	Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.
Expiration Date	12 months
Shipping	Ice bag